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Data cited in "Lung protective ventilation does not impact left ventricular output or molecular composition in a lamb model of preterm birth" manuscript

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posted on 2024-07-25, 03:51 authored by Prudence PereiraPrudence Pereira

  

Rationale: Preterm birth occurs before the cardiorespiratory systems are fully developed to support ex-utero life. The primary aim of lung protective ventilation is to support gas exchange whilst minimising lung injury. However, few studies have examined the impact of lung protective ventilation on the preterm heart.

Objective:To determine the impact of different lung protective ventilation strategies on left ventricular output (LVO) and proteome expression in a preterm lamb model.

Methods: Preterm lambs (123-127d gestation, term ~145d) were allocated to receive one of three lung protective ventilation strategies for 15 minutes from birth. Doppler echocardiography was used to evaluate LVO. Quantitative mass spectrometry-based proteomics identified changes in protein expression in left ventricular tissue. Gene ontology was used to categorise the biological process and cellular location of altered proteins. 

Results:LVO was comparable between strategies. 5,218 proteins were quantified in left ventricular tissue samples with 0.3-0.5% of proteins significantly altered between lung protective ventilation strategies. Biological process assignment of differentially expressed proteins was largely similar in both strategies. 

Conclusion: In the immediate post-birth period, lung protective ventilation strategies utilising either a dynamic PEEP with static tidal volume ( VT) or Dynamic PEEP with graded VT did not significantly impact LVO and altered <0.5% of proteins when compared with a standard ventilation protocol of static PEEP and static VT. This suggests that lung protective ventilation strategies initiated in the delivery room utilising these approaches are not impacting left heart function.  

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