Imaging data for Dorison et al., 2022

 

Imaging data for the manuscript "Kidney organoids generated using an allelic series of NPHS2 point variants reveal distinct intracellular Podocin mistrafficking"

dataset.zip (29.8GB uncompressed): Raw imaris (.ims) images taken using Andor Dragonfly Spinning Disk Confocal. Organised into subfolders by subcellular stain and then order of channels. Also contains a .txt file with metadata for every image.

edited_ROIs.zip (8.46GB uncompressed): Podocyte region of interest for each image as TIFF files (.tiff), manually proofread by an expert human annotator (Aude Dorison).

imageJ_rollingball.zip (32.4GB uncompressed): Relevant channels of each raw image with background subtraction (using rolling ball method with radius of 50 pixels) processed using Fiji/ImageJ as TIFF files (.tiff). This is used for downstream processing.

Code is available at https://github.com/KidneyRegeneration/Dorison2022/